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Shell middens are hard to define because they vary so greatly in size, content and form, but they are generally considered to be “a cultural deposit of which the principal component is shell” (, p. These deposits are especially found after the establishment of modern sea level in the mid-Holocene, and have been recorded in their hundreds of thousands around the coastlines of the world, often forming large mounds containing many millions of shells.

Earlier deposits are much less frequent, most probably due to Holocene sea level rise, resulting in the submergence of palaeoshorelines and the associated archaeological evidence ().

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This protocol provides a preliminary test for the presence of an intracrystalline fraction of proteins (by bleaching experiments and subsequent heating at high temperature), checking the closed system behaviour of this fraction during diagenesis.This means that the amino acid can have two different configurations, "D" or "L" which are mirror images of each other.With a few important exceptions, living organisms keep all their amino acids in the "L" configuration.Only species which pass both tests can be considered suitable for further studies to obtain reliable age information.This amino acid geochronological technique is also applied to midden deposits at two latitudinal extremes: Northern Scotland and the Southern Red Sea.For example, a shoe is chiral: you cannot turn a left-foot shoe into a right-foot shoe by turning it round or flipping it over.